Target
Human KCNJ1 / ROMK
Synonyms
KCNJ1 | KIR1.1 | ROMK1 | ROMK3 | ROMK5 | Inwardly rectifying K+ channel | ROMK | Romk2 | ROMK4
Reactivity
Human, Monkey, Bovine, Horse, Pig
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DQININFVVDAGNEN, from the internal region of the protein sequence according to NP_000211.1NP_722448.1.
Specificity
Human KCNJ1 / Kir1.1. This antibody is expected to recognize reported isoforms NP_722449.2 and NP_000211. The following reported variants represent identical protein: NP_722451.1, NP_722449.2, NP_722450.1, NP_722448.1.
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 45kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 44.8kDa according to NP_000211.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee