Human ITGB5 / Integrin Beta 5

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

CHO cell-derived rhIntegrin b5 extracellular domain.

Extracellular Domain

Recognizes human Integrin b5 extracellular domain. In Direct ELISAs and Western Blots, this antibody shows less than 5% cross-reactivity with rhIntegrin b1, rhIntegrin b3, rhIntegrin b4, rhIntegrin b6, and rmIntegrin b7. Species sequence Homology: mouse, 91%, canine, 89%.

• ICC (10 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• Flow Cytometry
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 0.1-0.2 ug/ml. Detection limit ~5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Immunocytochemistry: 10 ug/ml to detect b5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. Detection limit ~0.2 ng/well.

Lyophilized PBS, 5% Trehalose

Reconstitute in sterile PBS.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P18084 NCBI: NM_002213 NP_002204.2