Target
Human ITGB1 / Integrin Beta 1 / CD29
Synonyms
ITGB1 | CD29 | CD29 antigen | Integrin beta-1 | MDF2 | MSK12 | Integrin VLA-4 beta subunit | VLA-BETA | VLAB | Beta 1 integrin | FNRB | GPIIA | Integrin Beta 1 | VLA-4 subunit beta
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Jurkat T-leukemic cell line
Specificity
Recognizes human integrin b1 subunit. Recognizes transfectant cells displaying human integrin b1 and purified integrin b1.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Western blot
- Immunoprecipitation
- Flow Cytometry
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B3164 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3164 was determined to be 10 ug/ml. Function: Stimulates adhesion of cells to extracellular matrix proteins. Does not bind reduced integrin b1 on Western Blot.
Presentation
0.02 M PBS, pH 7.6, 0.1% sodium azide.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGB1 / Integrin Beta 1 / CD29