Target
Mouse ITGB1 / Integrin Beta 1 / CD29
Synonyms
ITGB1 | CD29 | CD29 antigen | Integrin beta-1 | MDF2 | MSK12 | Integrin VLA-4 beta subunit | VLA-BETA | VLAB | Beta 1 integrin | FNRB | GPIIA | Integrin Beta 1 | VLA-4 subunit beta
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
Integrin, beta1
Specificity
Reacts with b1 subunit of VLA (b1) integrins. Species cross-reactivity: mouse.
Applications
- IHC
- IHC - Frozen (20 µg/ml)
- Western blot (10 µg/ml)
- Immunoprecipitation
- Flow Cytometry (10 µg/ml)
- Functional Assay
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Usage
Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry and Western Blot. Not suitable for use in Inhibition. Immunoprecipitation: 2 ug Lysate of 1E6 cell. Flow Cytometry: 10 ug/ml. Immunohistochemistry (Frozen): 20 ug/ml. As a result of the wide tissue distribution of VLA (b1), binding to diverse cell types is commonly observed. Western Blot: 10 ug/ml. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples. Inhibition: Does not block VLA integrin mediated cell adhesion to matrix proteins. Positive control: Human OVCAR5 cell line whole cell lysate.
Presentation
PBS, pH 7.6, 0.25 M NaCl, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGB1 / Integrin Beta 1 / CD29