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Catalog Number Size Price
LS-C661882-10 10 µg $318 
LS-C661882-100 100 µg $470 
ITGAL / CD11a Antibody - IHC analysis of CD11a using anti-CD11a antibody. CD11a was detected in immunocytochemical section of U937 Cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD11a Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ITGAL / CD11a Antibody - Western blot - Anti-CD11a Picoband Antibody
ITGAL / CD11a Antibody - Flow Cytometry analysis of U937 cells using anti-CD11a antibody. Overlay histogram showing U937 cells stained with anti-CD11a antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11a Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ITGAL / CD11a Antibody - IHC analysis of CD11a using anti-CD11a antibody. CD11a was detected in immunocytochemical section of U937 Cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD11a Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ITGAL / CD11a Antibody - Western blot - Anti-CD11a Picoband Antibody
ITGAL / CD11a Antibody - Flow Cytometry analysis of U937 cells using anti-CD11a antibody. Overlay histogram showing U937 cells stained with anti-CD11a antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11a Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human ITGAL / CD11a Antibody (IHC, WB) LS‑C661882

Polyclonal Rabbit anti‑Human ITGAL / CD11a Antibody (IHC, WB) LS‑C661882

Antibody:
ITGAL / CD11a Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661882-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ITGAL / CD11a Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD11a antibody LS-C661882 is an unconjugated rabbit polyclonal antibody to human CD11a (ITGAL). Validated for Flow, ICC, IHC and WB.
Target
Human ITGAL / CD11a
Synonyms
ITGAL | CD11A | Integrin alpha-L | LFA-1 alpha | LFA-1A | Integrin gene promoter | Alpha polypeptide) | CD11a antigen | LFA-1 | LFA1A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CD11a recombinant protein (Position: F161-L349). Human CD11a shares 73.7% mino acid (aa) sequence identity with mouse CD11a.
Specificity
Leukocytes.
Applications
  • IHC
  • ICC
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGAL / CD11a
P20701 NM_002209 NP_002200.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024