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Catalog Number Size Price
LS-C757842-200 200 µl $393 
ISG15 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling ISG15 with ISG15 Antibody (N-term)) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
ISG15 Antibody - All lanes: Anti-ISG15 Antibody (N-term)) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
ISG15 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling ISG15 with ISG15 Antibody (N-term)) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
ISG15 Antibody - All lanes: Anti-ISG15 Antibody (N-term)) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Monoclonal Mouse anti‑Human ISG15 Antibody (clone 1031CT2.8.3, N‑Terminus, IF, WB) LS‑C757842

Monoclonal Mouse anti‑Human ISG15 Antibody (clone 1031CT2.8.3, N‑Terminus, IF, WB) LS‑C757842

Antibody:
ISG15 Mouse anti-Human Monoclonal (N-Terminus) (1031CT2.8.3) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757842-200
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ISG15 Mouse anti-Human Monoclonal (N-Terminus) (1031CT2.8.3) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ISG15 antibody LS-C757842 is an unconjugated mouse monoclonal antibody to human ISG15 (N-Terminus). Validated for ELISA, IF and WB.
Target
Human ISG15
Synonyms
ISG15 | HUCRP | IFI15 | ISG15 ubiquitin-like modifier | G1P2 | IP17 | Ubiquitin-like protein ISG15 | UCRP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1031CT2.8.3
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
This ISG15 antibody is generated from a mouse immunized with a recombinant protein from human ISG15.
Epitope
N-Terminus
Applications
  • Immunofluorescence (1:25)
  • Western blot (1:8000)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 17.9 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ISG15
P05161 NM_005101 NP_005092.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/16/2024