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Catalog Number Size Price
LS-C777304-100 100 µg (1 mg/ml) $477 
IRGM / LRG-47 Antibody - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody. Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
IRGM / LRG-47 Antibody - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody. Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
IRGM / LRG-47 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.
IRGM / LRG-47 Antibody - Rabbit Anti-IRGM Antibody used in Western blot (WB) on HeLa and HEK293Trap cell lysates
IRGM / LRG-47 Antibody - Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Rabbit Anti-IRGM Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~20.1 kDa.
IRGM / LRG-47 Antibody - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody. Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
IRGM / LRG-47 Antibody - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody. Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
IRGM / LRG-47 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.
IRGM / LRG-47 Antibody - Rabbit Anti-IRGM Antibody used in Western blot (WB) on HeLa and HEK293Trap cell lysates
IRGM / LRG-47 Antibody - Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Rabbit Anti-IRGM Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~20.1 kDa.
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Polyclonal Rabbit anti‑Human IRGM / LRG‑47 Antibody (Biotin, IHC, IF, WB) LS‑C777304

Polyclonal Rabbit anti‑Human IRGM / LRG‑47 Antibody (Biotin, IHC, IF, WB) LS‑C777304

Antibody:
IRGM / LRG-47 Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$477
LS-C777304-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IRGM / LRG-47 Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
LRG-47 antibody LS-C777304 is a biotin-conjugated rabbit polyclonal antibody to human LRG-47 (IRGM). Validated for IF, IHC and WB.
Target
Human IRGM / LRG-47
Synonyms
IRGM | LRG-47-like protein | IFI1 | IRGM1 | LRG47 | LRG-47
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available conjugated with Atto 390, Atto 488, Atto 594, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of Human IRGM
Specificity
Detects ~20 kDa.
Applications
  • IHC (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IRGM / LRG-47
A1A4Y4 NP_001139277.1

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Requested From: United States
Date Requested: 11/2/2024