Target
Human INSR / Insulin Receptor
Synonyms
INSR | CD220 | ILP receptor | Insulin receptor | IR | HHF5 | CD220 antigen
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
IM-9 lymphocytes followed by purified insulin receptor. MW of Antigen: 135kD. Epitope: aa469-592 (exon 7/8). Cellular Localization: Cell membrane.
Specificity
Recognizes human Insulin receptor, alpha subunit. Inhibits insulin binding (~80%) and mimics insulin action. Weakly reacts with bovine, porcine and sheep. Does not react with rabbit, mouse and rat.
Applications
- Immunoprecipitation
- ELISA
- Functional Assay
Usage
Suitable for use in ELISA, Immunoprecipitation, Tyrosine Kinase Assay and Inhibition of insulin binding. May also be used as insulin-like agonist. ELISA: Both as capture and detection. Immunoprecipitation (Use Protein G): 2 ug/mg protein lysate. Tyrosine Kinase Assay: Antibody-mediated capture on microtiter plates. Positive control: IM-9 lymphocyte cells and Placenta. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
10 mM PBS, pH 7.4. Glycerol free.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About INSR / Insulin Receptor