Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C154121-100 100 µg (1 mg/ml) $552 
IL7 Antibody - Biotin Rabbit Anti-Human IL-7 Antibody - Western Blot. Western Blot showing detection of Human IL-7. 50 ng of Human IL-7 (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated - 209-406-B94 secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 17 kD molecular weight position expected for Human IL-7. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti‑Human IL7 Antibody (Biotin, IHC, WB) LS‑C154121

Polyclonal Rabbit anti‑Human IL7 Antibody (Biotin, IHC, WB) LS‑C154121

Antibody:
IL7 Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Biotin, Unmodified
Price
Catalog Number
$552
LS-C154121-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IL7 Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Biotin, Unmodified

Specifications

Description
IL7 antibody LS-C154121 is a biotin-conjugated rabbit polyclonal antibody to human IL7. Validated for ELISA, IHC and WB.
Target
Human IL7
Synonyms
IL7 | IL-7 | Interleukin-7 | Interleukin 7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Specificity
This antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.4 kD in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL7
P13232 NM_000880 NP_000871.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024