Target
Human IL6R / IL6 Receptor
Synonyms
IL6R | CD126 | gp80 | IL-6R subunit alpha | IL-6R-alpha | IL-6RA | IL6Q | IL-6 receptor subunit alpha | IL-6R 1 | IL-6R-1 | Interleukin 6 receptor | Membrane glycoprotein 80 | CD126 antigen | IL6RA | IL6RQ
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Sf 21-derived rhIL-6R.
Specificity
Recognizes human IL-6R.
Applications
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (25 µg/ml)
- ELISA
- Neutralization
Usage
Suitable for use in Neutralization, Western Blot, ELISA and Flow Cytometry. Neutralization of Human IL6 R Bioactivity: ~0.1-0.2 ug/ml in the presence of 20 ng/ml of rhIL-6 R and 20 ng/ml of rhIL-6, using the M1 cell line. Western Blot: 1-2 ug/ml; The detection limit is ~100 ng/lane under non-reducing and reducing conditions. ELISA Capture: 2-8 ug/ml. Use biotinylated human IL-6 R detection antibody and recombinant human IL-6 R as the standard. Flow Cytometry: 25 ug/ml; 10 ul labels 1-2.5x10^5 U937 cells in a total reaction volume <200ul.
Presentation
Lyophilized PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL6R / IL6 Receptor