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| Catalog Number | Size | Price |
|---|---|---|
| LS-C7917-500 | 500 µg | $523 |
Monoclonal Rat anti‑Mouse IL5 Antibody (IF, WB) LS‑C7917
Monoclonal Rat anti‑Mouse IL5 Antibody (IF, WB) LS‑C7917
Antibody:
IL5 Rat anti-Mouse Monoclonal Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
IL5 Rat anti-Mouse Monoclonal Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Specifications
Description
IL5 antibody LS-C7917 is an unconjugated rat monoclonal antibody to IL5 from mouse. It is reactive with human and mouse. Validated for ELISA, Flow, IF and WB.
Target
Mouse IL5
Synonyms
IL5 | EDF | Interleukin-5 | IL-5 | T-cell replacing factor | TRF
Host
Rat
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Semi-purified mouse T cell clone supernatant.
Specificity
Recognizes mouse and human Interleukin-5 (IL-5).
Applications
- Immunofluorescence
- Western blot
- Flow Cytometry
- ELISA
Usage
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions.
Presentation
100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL5
Publications (0)
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Featured Products
Species:
Human
Applications:
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Mouse, Human, Guinea pig
Applications:
Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/15/2025
Date Requested: 1/15/2025
