Target
Human IL5
Synonyms
IL5 | EDF | Interleukin-5 | IL-5 | T-cell replacing factor | TRF
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Preservative Free
Immunogen
Recombinant Human IL-5/IL5/Interleukin 5 protein
Specificity
Human IL-5. Has cross-reactivity with mouse IL5 in ELISA assay
Applications
- ELISA
- Blocking
- Neutralization
Usage
ELISA(Det): This antibody will detect Human IL-5/IL5 / Interleukin 5 in ELISA pair set. Block: In a functional ELISA which immobilized Human IL5 at 0.4 ug/ml (100 µL/well) in the plate, the Rabbit anti-Human IL5 Monoclonal Antibody can block the binding of 2 ug/ml Human IL5R to human IL5, the EC50 is 0.23 ug/ml. Neutralization: The neutralization activity of IL5 neutralizing antibody is measured by its ability to neutralize IL5-induced proliferation in the TF-1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 2-8 ng/mL in the presence of 1. 25 ng/mL Human IL5. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Presentation
0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee