Mouse IL5

Rat

Mouse, Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Purified

Azide-free

Semi-purified mouse T cell clone supernatant.

Recognizes mouse and human Interleukin-5 (IL-5).

• Immunofluorescence
• Western blot
• Flow Cytometry
• ELISA
• Neutralization

ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing mouse IL-5 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, No preservatives added

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05113 NCBI: NM_000879 NP_000870.1