Usage
ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-3 protein levels.2,3,5 Biotinylated antibody should be paired with purified antibody (Cat. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-3 under reducing conditions.
Presentation
100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee