Mouse IL25 / IL17E

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

E. coli-derived rmIL-17E.

Recognizes mouse IL-17E. In sandwich ELISAs no cross-reactivity or interference was observed with rhIL-17E, rmIL-17, rmIL-17B, C, D, or F.

• Flow Cytometry
• ELISA

Suitable for use in ELISA and Flow Cytometry. ELISA (Capture): 2-8 ug/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard. Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25 ug/ml and add 10 ul of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome.

Lyophilized from PBS, 5% Trehalose

40-50% glycerol, PBS

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9H293 NCBI: NM_022789 NP_073626.1