Target
Human IL23A / IL-23 p19
Synonyms
IL23A | IL-23 subunit alpha | IL-23A | Interleukin 23 p19 subunit | Interleukin-23 subunit alpha | IL23P19 | Interleukin-23 subunit p19 | p19 | SGRF | IL-23 | IL-23 p19 | IL-23-A | IL-23p19
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Affinity purified
Applications
- Western blot
- ELISA
- Sandwich ELISA
Usage
The 473P19 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-23 (p19p40) protein levels in combination with the biotinylated (p40-specific) C8.6 antibody for detection and recombinant human IL-23 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2.5-8.0 ug/ml. A standard curve consisting of doubling dilutions of the recombinant IL-23 standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. TMB, rather than ABTS, should be used as the substrate. The antibody 473p19 also works in immunoblotting under both reducing and non reducing conditions at 2 ug/ml.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee