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Catalog Number	Size	Price
LS-C662800-10	10 µg	$318
LS-C662800-100	100 µg	$470

IL18BP Antibody - Western blot analysis of IL18 binding protein using anti-IL18 binding protein antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse heart tissue lysate,Lane 2: mouse brain tissue lysate,Lane 3: rat heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 binding protein antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL18 binding protein at approximately 43KD. The expected band size for IL18 binding protein is at 21KD.

IL18BP Antibody - Western blot - Anti-IL18 binding protein antibody

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Polyclonal Rabbit anti‑Mouse IL18BP Antibody (WB) LS‑C662800

Name: Anti-IL18BP Antibody | Rabbit anti-Mouse Polyclonal ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662800-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

IL18BP Rabbit anti-Mouse Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$318

LS-C662800-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IL18BP Rabbit anti-Mouse Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

IL18BP antibody LS-C662800 is an unconjugated rabbit polyclonal antibody to mouse IL18BP. Validated for ELISA and WB.

Target

Mouse IL18BP

Synonyms

See All IL18BP Antibodies

Host

Rabbit

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Modifications

Unmodified

Immunogen

E. coli-derived mouse IL18 binding protein recombinant protein (Position: T29-A193).

Applications

Western blot
ELISA

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IL18BP

Uniprot: O95998 NCBI: NM_173042 NP_766630.2