Target
Human IL18
Synonyms
IL18 | IGIF | IL-1g | Interleukin-18 | IFN-gamma-inducing factor | IL-1 gamma | IL-18 | Iboctadekin | IL1F4 | Interleukin-1 gamma
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-NPPDNIKDTKSDI, from the internal region of the protein sequence according to NP_001553.1NP_001230140.1.
Specificity
Human IL18 / IL-18. This antibody is expected to recognise both reported isoforms (NP_001553.1; NP_001230140.1).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 23kDa band observed in Human Placenta lysates and in lysates of cell line HeLa (calculated MW of 22.3kDa according to NP_001553.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee