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Catalog Number Size Price
LS-C154151-100 100 µg (1 mg/ml) $552 
IL17A Antibody - Biotin Rabbit Anti-Rat IL-17A Antibody - Western Blot. Western Blot showing detection of Rat IL-17A. 50 ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated - 212-406-B32 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 15 kD molecular weight position expected for Rat IL-17A. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti‑Rat IL17A Antibody (Biotin, IHC, WB) LS‑C154151

Polyclonal Rabbit anti‑Rat IL17A Antibody (Biotin, IHC, WB) LS‑C154151

Antibody:
IL17A Rabbit anti-Rat Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rat, Mouse
Format:
Biotin, Unmodified
Price
Catalog Number
$552
LS-C154151-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IL17A Rabbit anti-Rat Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rat, Mouse
Format:
Biotin, Unmodified

Specifications

Description
IL17A antibody LS-C154151 is a biotin-conjugated rabbit polyclonal antibody to IL17A from rat. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.
Target
Rat IL17A
Synonyms
IL17A | CTLA-8 | CTLA8 | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Host
Rabbit
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Antiserum
Modifications
Unmodified
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.
Specificity
This antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 30.0 kD in size corresponding to rat IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL17A
Q16552 NM_002190 NP_002181.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/24/2024