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Catalog Number Size Price
LS-C662181-10 10 µg $318 
LS-C662181-100 100 µg $470 
IL15RA Antibody - Immunohistochemistry - Anti-IL15RA Picoband Antibody
IL15RA Antibody - Immunohistochemistry - Anti-IL15RA Picoband Antibody
IL15RA Antibody - Western blot analysis of IL15RA using anti-IL15RA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL15RA antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL15RA at approximately 28KD. The expected band size for IL15RA is at 28KD.
IL15RA Antibody - Western blot - Anti-IL15RA Picoband Antibody
IL15RA Antibody - Immunohistochemistry - Anti-IL15RA Picoband Antibody
IL15RA Antibody - Immunohistochemistry - Anti-IL15RA Picoband Antibody
IL15RA Antibody - Western blot analysis of IL15RA using anti-IL15RA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL15RA antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL15RA at approximately 28KD. The expected band size for IL15RA is at 28KD.
IL15RA Antibody - Western blot - Anti-IL15RA Picoband Antibody
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Polyclonal Rabbit anti‑Human IL15RA Antibody (IHC, WB) LS‑C662181

Polyclonal Rabbit anti‑Human IL15RA Antibody (IHC, WB) LS‑C662181

Antibody:
IL15RA Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662181-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IL15RA Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL15RA antibody LS-C662181 is an unconjugated rabbit polyclonal antibody to human IL15RA. Validated for ELISA, IHC and WB.
Target
Human IL15RA
Synonyms
IL15RA | CD215 antigen | IL-15RA | IL-15 receptor subunit alpha | IL-15R-alpha | Interleukin 15 receptor, alpha | CD215
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human IL15RA recombinant protein (Position: I31-T174).
Specificity
Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL15RA
Q13261 NM_002189 NP_002180.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/1/2024