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| Catalog Number | Size | Price |
|---|---|---|
| LS-C130256-100 | 100 µg | $962 |
Polyclonal Goat anti‑Dog IL12 Antibody (WB) LS‑C130256
Polyclonal Goat anti‑Dog IL12 Antibody (WB) LS‑C130256
Antibody:
IL12 Goat anti-Dog Polyclonal Antibody
Application:
WB, ELISA, Neut
Reactivity:
Dog
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
IL12 Goat anti-Dog Polyclonal Antibody
Application:
WB, ELISA, Neut
Reactivity:
Dog
Format:
Unconjugated, Unmodified
Specifications
Description
IL12 antibody LS-C130256 is an unconjugated goat polyclonal antibody to dog IL12. Validated for ELISA, Neut and WB.
Host
Goat
Reactivity
Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
CHO-derived recombinant canine interleukin 12 p40 (aa 23-329, Q28268) and p35 (aa 26-222, Q28267).
Specificity
This antibody recognizes both the p35 and p40 sub-units of canine IL-12. Cross-reactivity (direct ELISA): ~40% with recombinant human IL-12 p35/p40 and recombinant feline IL-12 p35/p40; <10% with recombinant rat IL-12 p35/p40.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA
- Neutralization
Usage
Suitable for use in ELISA, Western Blot and Neutralization. ELISA (capture): 0.2-0.8 ug/ml. Western Blot: 0.1-0.2 ug/ml. Detection limit for rcaIL-12 is ~50 ng/lane under non-reducing and reducing conditions. Neutralization: The ND 50 was determined to be ~0.1-0.5 ug/ml in the presence of 25 ng/ml of rcaIL-12, using PHA activated human peripheral blood mononuclear cells (PBMCs).
Presentation
Lyophilized from Sterile Solution PBS, 5% Trehalose
Reconstitution
Sterile PBS, 40-50% glycerol
Storage
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Requested From: United States
Date Requested: 11/24/2024
Date Requested: 11/24/2024
