Human IL12

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein A purified

Unmodified

CHO derived recombinant Human IL-12.

Human IL-12

• Western blot (0.5 - 1 µg/ml)
• ELISA (2 - 4 µg/ml)

Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 10 ug/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human IL-12 when used with biotinylated antigen affinity purified anti-human IL-12 (LS-C104425) as the detection antibody at a concentration of approximately 4-8 ug/ml. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1 ng/lane, under non-reducing conditions.

Lyophilized from PBS

Reconstitute with sterile water to a concentration of 1 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.