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Catalog Number Size Price
LS-C801182-100 100 µl (1 mg/ml) $379 
LS-C801182-200 200 µl (1 mg/ml) $421 
IL10RA Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
IL10RA Antibody - Western blot analysis of Phospho-IL-10R alpha (Tyr496) antibody expression in HuvEc cells lysates. The lane on the right is treated with the antigen-specific peptide.
IL10RA Antibody - Western blot analysis of IL-10R alpha phosphorylation expression in HuvEc whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
IL10RA Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
IL10RA Antibody - Western blot analysis of Phospho-IL-10R alpha (Tyr496) antibody expression in HuvEc cells lysates. The lane on the right is treated with the antigen-specific peptide.
IL10RA Antibody - Western blot analysis of IL-10R alpha phosphorylation expression in HuvEc whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human IL10RA Antibody (phospho‑Tyr496, IF, WB) LS‑C801182

Polyclonal Rabbit anti‑Human IL10RA Antibody (phospho‑Tyr496, IF, WB) LS‑C801182

Antibody:
IL10RA Rabbit anti-Human Polyclonal (pTyr496) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801182-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IL10RA Rabbit anti-Human Polyclonal (pTyr496) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL10RA antibody LS-C801182 is an unconjugated rabbit polyclonal antibody to human IL10RA (pTyr496). Validated for IF, Peptide-ELISA and WB.
Target
Human IL10RA
Synonyms
IL10RA | CD210 | CD210a | CD210 antigen | IBD28 | IL-10R subunit 1 | Il-10r | IL-10R subunit alpha | Interleukin 10 receptor, alpha | IL10R | Interleukin-10 receptor | HIL-10R | CDW210A | IL-10 receptor subunit alpha | IL-10R1 | IL-10RA | Interleukin-10 receptors | IL-10 RA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human IL-10R alpha around the phosphorylation site of Tyrosine 496.
Epitope
pTyr496
Specificity
Phospho-IL-10R alpha (Tyr496) Antibody detects endogenous levels of IL-10R alpha only when phosphorylated at Tyrosine 496.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL10RA
Q13651 NM_001558 NP_001549.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024