Target
Mouse ZBTB7B / HcKrox
Synonyms
ZBTB7B | C-KROX | HcKROX | THPOK | ZFP-67 | Zinc finger protein 67 homolog | Zinc finger protein Th-POK | ZNF857B | Zinc finger protein 857B | ZFP67 | ZBTB15
Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Predicted
Human, Rat (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of mouse cKrox protein.
Specificity
This antibody is directed against mouse cKrox. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with cKrox from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
- IHC
- IHC - Paraffin (1:100)
- Western blot (1:500 - 1:3000)
- ELISA (1:2500 - 1:10000)
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Usage
This antibody has been tested for use in ELISA, Immunohistochemistry with FFPE human tissues and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kD in size corresponding to cKrox by western blotting in the appropriate cell lysate or extract. Lysates from mouse thymocytes are recommended for use as positive controls.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee