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| Catalog Number | Size | Price |
|---|---|---|
| LS-B436-50 | 50 µg (1 mg/ml) | $515 |
![WRNIP1 / WHIP Antibody - Anti-Human WHIP Antibody - Western Blot. Western blot analysis is shown using anti-Human WHIP antibody with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1. ~30 ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kD is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye 800 labeled Goat-a-Rabbit IgG [H&L] MX10 ( diluted 1:5000 for 45 min. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-wrnip1-whip-antibody-ls-b436/62868_1476648.jpg)
![WRNIP1 / WHIP Antibody - Anti-Human WHIP Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate. ~30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kD is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye 800 labeled Goat-a-Rabbit IgG [H&L] MX10 ( diluted 1:5000 for 45 min. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-wrnip1-whip-antibody-ls-b436/62867_119388.jpg)
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IHC‑plus™ Polyclonal Rabbit anti‑Human WRNIP1 / WHIP Antibody (IHC, WB) LS‑B436
IHC‑plus™ Polyclonal Rabbit anti‑Human WRNIP1 / WHIP Antibody (IHC, WB) LS‑B436
Note: This antibody replaces LS-C19088
Antibody:
WRNIP1 / WHIP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
WRNIP1 / WHIP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
WHIP antibody LS-B436 is an unconjugated rabbit polyclonal antibody to WHIP (WRNIP1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human WRNIP1 / WHIP
Synonyms
WRNIP1 | Putative helicase RUVBL | WHIP | RP11-420G6.2 | ATPase WRNIP1 | BA420G6.2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA (1:10000 - 1:40000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B436 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B436 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About WRNIP1 / WHIP
Validation
Anti-WRNIP1 / WHIP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-WRNIP1 / WHIP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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LSBio Ratings
IHC-plus™ WRNIP1 / WHIP Antibody for IHC, WB/Western, ELISA LS-B436 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
