Human VTN / Vitronectin

Mouse

Human, Bovine, Cat, Dog, Goat, Horse (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Affinity purified

Unmodified

Human vitronectin purified from plasma by heparin-affinity chromatography.

Recognizes human vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Species Crossreactivity: feline, canine, goat, bovine and to a lesser extent with equine. Binds nearly as well to native vitronectin as to denatured. Is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:50)
• ELISA (1:30000)

Immunohistochemistry: LS-B2118 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2118 was determined to be 2.5 ug/ml. ELISA: 1:15000. Coat with vitronectin directly onto the microtiter well. Please note that cross-reactivity with species other than human has not been confirmed in IHC at this time. In the absence of testing data we cannot guarantee cross-reactivity with non-human species in IHC.

PBS, pH 7.4, 15 mM Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04004 NCBI: NM_000638 NP_000629.3