Human UBE2N / UBC13

Goat

Human (tested or 100% immunogen sequence identity)

Chimpanzee, Mouse, Rat, Bovine, Dog, Chicken, Zebrafish, Macaque, Xenopus laevis, Xenopus tropicalis (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-55 of human UBC13 protein.

A BLAST analysis was used to suggest cross reactivity with UBC13 protein from a broad range of sources including human, dog, bovine, Xenopus laevis, Xenopus tropicalis, , zebrafish, rat, mouse, chimpanzee, macaque and chicken based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:500 - 1:2000)
• ELISA (1:5000 - 1:25000)

Immunohistochemistry: LS-B433 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B433 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P61088 NCBI: NM_003348 NP_003339.1