Human TRAF6

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the C-Terminus of the human and mouse TRAF6 (TNF receptor-associated factor 6) protein.

C-Terminus

Recognizes human TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids).

• IHC
• IHC - Paraffin (1:200 - 1:400)
• Western blot (1 - 3 µg/ml)
• Immunoprecipitation

Immunohistochemistry: LS-B190 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B190 was determined to be 2.5 ug/ml. Immunoprecipitation: 7 ug/reaction. Positive control: Human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates. Please note that cross-reactivity of this antibody with mouse has been confirmed only in WB. The antibody has not been evaluated for use in IHC with mouse samples at this time and suitability cannot be guaranteed.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9Y4K3 NCBI: NM_004620 NP_004611.1