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| Catalog Number | Size | Price |
|---|---|---|
| LS-B315-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human TP53BP1 / 53BP1 Antibody (IHC, WB) LS‑B315
IHC‑plus™ Polyclonal Rabbit anti‑Human TP53BP1 / 53BP1 Antibody (IHC, WB) LS‑B315
Note: This antibody replaces LS-C2590
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
53BP1 antibody LS-B315 is an unconjugated rabbit polyclonal antibody to 53BP1 (TP53BP1) from human. It is reactive with human and mouse. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TP53BP1 / 53BP1
Synonyms
TP53BP1 | 53BP1 | p202 | p53-binding protein 1 | p53-binding protein 53bp1 | p53BP1
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide, which represented a portion of human tumor protein p53-binding protein 1 encoded in exon 11, 12 and 19 (LocusLink ID 7158).
Specificity
human 53BP1 protein.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ICC (1:100 - 1:1000)
- Western blot (1:1000 - 1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B315 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B315 was determined to be 5 ug/ml.
Presentation
Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide
Storage
Store at 4°C. Avoid freezing.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TP53BP1 / 53BP1
Validation
Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ TP53BP1 / 53BP1 Antibody for IHC, ICC, WB/Western LS-B315 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Goat, Fish, Primate
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse
Applications:
IHC, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC - Paraffin, Immunoprecipitation
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
