Human THBS1 / Thrombospondin-1

Mouse

Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

A6.1

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Azide-free

Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP

Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.

• IHC
• IHC - Paraffin (10 µg/ml)
• Immunofluorescence
• Western blot
• Immunoprecipitation
• Flow Cytometry

Immunohistochemistry: LS-B2570 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2570 was determined to be 10 ug/ml. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, pH 7.4

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07996 NCBI: NM_003246 NP_003237.2