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Catalog Number	Size	Price
LS-B16563-100	100 µg	$485

SNAP25 Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

SNAP25 Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

SNAP25 Antibody - Western blot analysis of SNAP25 using anti-SNAP25 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human U-87MG whole cell lysate,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 25KD. The expected band size for SNAP25 is at 25KD.

SNAP25 Antibody - Flow Cytometry analysis of U20S cells using anti-SNAP25 antibody. Overlay histogram showing U20S cells stained with anti-SNAP25 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

SNAP25 Antibody - Flow Cytometry analysis of U87 cells using anti-SNAP25 antibody. Overlay histogram showing U87 cells stained with anti-SNAP25 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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IHC‑plus™ Polyclonal Rabbit anti‑Human SNAP25 Antibody (IHC, WB) LS‑B16563

Name: IHC-plus™ SNAP25 Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16563-100
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C756499

Antibody:

SNAP25 Rabbit anti-Human Polyclonal Antibody

Application:

IHC-P, ICC, WB, Flo, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$485

LS-B16563-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SNAP25 Rabbit anti-Human Polyclonal Antibody

Application:

IHC-P, ICC, WB, Flo, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

SNAP25 antibody LS-B16563 is an unconjugated rabbit polyclonal antibody to SNAP25 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human SNAP25

Synonyms

SNAP25 | BA416N4.2 | DJ1068F16.2 | SNAP | SUP | Super protein | RIC4 | SEC9 | SNAP-25 | RIC-4

See All SNAP25 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived human SNAP25 recombinant protein (Position:M1-L203).

Specificity

No cross reactivity with other proteins.

Applications

IHC - Paraffin (1:100)
ICC
Western blot (0.1 - 0.5 µg/ml)
Flow Cytometry
ELISA (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.