Target
Human SMAD7
Synonyms
SMAD7 | CRCS3 | HSMAD7 | MAD homolog 7 | SMAD 7 | MADH7 | MADH8 | Mothers against DPP homolog 7 | Mothers against DPP homolog 8 | MAD homolog 8 | SMAD family member 7
Reactivity
Human, Monkey, Bat, Bovine, Dog
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Rabbit (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Amino acids 12-29 (RLWRSRAPGGEDEEEGAG) and 36-50 (ELRGEGATDSRAHGA) of human SMAD7. This sequence is 100% or highly conserved between human and multiple species. Percent identity by BLAST analysis: Human, Monkey, Bovine, Dog, Bat, Panda (100%); Mouse, Rat, Rabbit, Pig, Platypus (94%).
Specificity
Two synthetic peptides corresponding to aa 12-18 (RLWRSRAPGGEDEEEGAG) and amino acids 36-50 (ELRGEGATDSRAHGA) of human SMAD7; GenBank Accession no. AAB81354.1. This sequence is 100% or highly conserved between human and multiple species.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:500 - 1:1000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B120 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B120 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee