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IHC‑plus™ Monoclonal Mouse anti‑Human SMAD3 Antibody (clone 2C12, IHC, WB) LS‑B5455
IHC‑plus™ Monoclonal Mouse anti‑Human SMAD3 Antibody (clone 2C12, IHC, WB) LS‑B5455
Note: This antibody replaces LS-C133305
Antibody:
SMAD3 Mouse anti-Human Monoclonal (2C12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
SMAD3 Mouse anti-Human Monoclonal (2C12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
SMAD3 antibody LS-B5455 is an unconjugated mouse monoclonal antibody to human SMAD3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SMAD3
Synonyms
SMAD3 | HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | HSMAD3 | Mad protein homolog | SMAD 3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Immunogen
SMAD3 (NP_005893, 120 aa ~ 222 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysates, cell line lysates and the recombinant protein used as the immunogen.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus laevis, Xenopus tropicalis
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human
Applications:
Western blot, ELISA
Reactivity:
Human, Mouse, Rat
Range:
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024