Target
Human SLC5A1 / SGLT1
Synonyms
SLC5A1 | D22S675 | HSGLT1 | Na(+)/glucose cotransporter 1 | Na+/glucose cotransporter 1 | SGLT1 | NAGT | Sodium-glucose transporter 1 | Sodium/glucose cotransporter 1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide surrounding amino acid 594 of human SGLT-1.
Specificity
The antibody detects 73 kD SGLT-1 from human samples and in a lesser extent from mouse and rat samples. A ~50 kD cleavage fragment can also be detected in Jurkat cell lysate.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.5 - 4 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B3060 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3060 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.2, 0.01% Thimerosal, 30% Glycerol, 0.5% BSA
Storage
Short term: -20°C; Long term: -70°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee