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Catalog Number	Size	Price
LS-B10468-50	50 µg (0.5 mg/ml)	$485

SH3GL1 / EEN Antibody - Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

SH3GL1 / EEN Antibody - SH3GL1 antibody (0.3 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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IHC‑plus™ Polyclonal Goat anti‑Human SH3GL1 / EEN Antibody (aa261‑272, IHC, WB) LS‑B10468

Name: IHC-plus™ SH3GL1 / EEN Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B10468-50
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C139731

Antibody:

SH3GL1 / EEN Goat anti-Human Polyclonal (aa261-272) Antibody

Application:

IHC, IHC-P, WB, Peptide-ELISA

Reactivity:

Human, Monkey, Dog

Format:

Unconjugated, Unmodified

$485

LS-B10468-50

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

SH3GL1 / EEN Goat anti-Human Polyclonal (aa261-272) Antibody

Application:

IHC, IHC-P, WB, Peptide-ELISA

Reactivity:

Human, Monkey, Dog

Format:

Unconjugated, Unmodified

Specifications

Description

EEN antibody LS-B10468 is an unconjugated goat polyclonal antibody to EEN (SH3GL1) (aa261-272) from human. It is reactive with human, dog and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human SH3GL1 / EEN

Synonyms

See All SH3GL1 / EEN Antibodies

Host

Goat

Reactivity

Human, Monkey, Dog (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-EPFDLGEPEQSN, from the internal region of the protein sequence according to NP_003016.1.

Epitope

aa261-272

Specificity

Human SH3GL1 / Endophilin-A2.

Applications

IHC
IHC - Paraffin (5 µg/ml)
Western blot (0.3 - 1 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kD band observed in lysates of cell line K562 (calculated MW of 41.5kD according to NP_003016.1. The observed molecular weight corresponds to earlier findings in literature with different antibodies (So et al, Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2563-8.; PMID: 9122235). Recommended concentration: 0.3-1 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SH3GL1 / EEN

Uniprot: Q99961 NCBI: NM_003025 NP_003016.1