Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B10614-50 50 µg (1 mg/ml) $515 
SERPINB2 / PAI-2 Antibody - Anti-SERPINB2 / PAI-2 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
SERPINB2 / PAI-2 Antibody - Anti-PAI-2 antibody - Western Blot. Western blot of affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 ug of HeLa whole cell lysates. The band at ~42 kD (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:5000 dilution of HRP- conjugated Gt-a-Rabbit IgG (LS-C60865) for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers. Personal communication, Luca DAgostino, Temple University, Philadelphia, PA.
SERPINB2 / PAI-2 Antibody - Anti-SERPINB2 / PAI-2 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
SERPINB2 / PAI-2 Antibody - Anti-PAI-2 antibody - Western Blot. Western blot of affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 ug of HeLa whole cell lysates. The band at ~42 kD (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:5000 dilution of HRP- conjugated Gt-a-Rabbit IgG (LS-C60865) for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers. Personal communication, Luca DAgostino, Temple University, Philadelphia, PA.
1 of 2
2 of 2

IHC‑plus™ Polyclonal Rabbit anti‑Human SERPINB2 / PAI‑2 Antibody (Internal, IHC, WB) LS‑B10614

IHC‑plus™ Polyclonal Rabbit anti‑Human SERPINB2 / PAI‑2 Antibody (Internal, IHC, WB) LS‑B10614

Note: This antibody replaces LS-C154245
Antibody:
SERPINB2 / PAI-2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B10614-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SERPINB2 / PAI-2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
PAI-2 antibody LS-B10614 is an unconjugated rabbit polyclonal antibody to PAI-2 (SERPINB2) (Internal) from human. It is reactive with human and monkey. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SERPINB2 / PAI-2
Synonyms
SERPINB2 | Monocyte Arg-serpin | HsT1201 | PAI | PLANH2 | Serpin B2 | PAI-2 | PAI2
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Primate (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal area of human PAI-2 protein.
Epitope
Internal
Specificity
A BLAST analysis was used to suggest cross reactivity with PAI-2 proteins from several primates based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 42-46.6 kD in size corresponding to PAI-2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPINB2 / PAI-2
P05120 NM_002575 NP_002566.1

Validation

SERPINB2 / PAI-2 Antibody - Anti-SERPINB2 / PAI-2 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SERPINB2 / PAI-2 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SERPINB2 / PAI-2 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ SERPINB2 / PAI-2 Antibody (Internal) for IHC, WB/Western, ELISA LS-B10614 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024