Human SELP / P-Selectin / CD62P

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Psel.KO.2.5

Unconjugated

Affinity purified

Unmodified

CD62P transfected 300.19 cells

Recognizes the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

• IHC
• IHC - Paraffin (10 µg/ml)
• Immunoprecipitation
• Flow Cytometry (1:1 - 1:50)

Immunohistochemistry: LS-B3656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3656 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

PBS, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_003005 NP_002996.2