Human SDC2 / Syndecan 2

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Affinity purified

Unmodified

Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).

Internal

Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 3 µg/ml)
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B2981 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2981 was determined to be 5 ug/ml.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P34741 NCBI: J04621 AAA52701.1