Human SCNN1G / ENaC Gamma

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-SNQLTDTQMLDE, from the C-Terminus of protein sequence according to NP_001030.2.

aa637-648

Human SCNN1G.

• IHC
• IHC - Paraffin (4 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:16000)

Immunohistochemistry: LS-B3024 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3024 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 140 kD band in Human Breast cancer and Colon lysates after 1 ug/ml antibody staining. We cannot find an explanation in the literature for the band we observe given the calculated size of 74.3kD according to NP_001030.2. The 140kD band was successfully blocked by incubation with the immunizing peptide.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P51170 NCBI: NM_001039 NP_001030.2