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IHC‑plus™ Monoclonal Mouse anti‑Human S100A8 / MRP8 Antibody (clone CF‑145, FITC, IHC, IF, WB) LS‑B2836
IHC‑plus™ Monoclonal Mouse anti‑Human S100A8 / MRP8 Antibody (clone CF‑145, FITC, IHC, IF, WB) LS‑B2836
Note: This antibody replaces LS-C62798
Antibody:
S100A8 / MRP8 Mouse anti-Human Monoclonal (FITC) (CF-145) Antibody
Application:
IHC-P, IF, WB, Flo
Overview
Antibody:
S100A8 / MRP8 Mouse anti-Human Monoclonal (FITC) (CF-145) Antibody
Application:
IHC-P, IF, WB, Flo
Specifications
Description
MRP8 antibody LS-B2836 is an FITC-conjugated mouse monoclonal antibody to human MRP8 (S100A8). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human S100A8 / MRP8
Synonyms
S100A8 | 60B8AG | CFAG | CAGA | Calgranulin A | Calgranulin-A | Calprotectin L1L subunit | Cystic fibrosis antigen | CGLA | L1Ag | NIF | p8 | Protein S100-A8 | MRP8 | Urinary stone protein band A | CP-10 | MA387 | MRP-8
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Purification
Protein G purified
Immunogen
Purified granulocyte antigen of human origin
Specificity
Anti-Human Calgranulin A monoclonal antibody reacts with the human Calgranulin A protein. Calgranulin A, also known as MRP-8 and S 100A8 is a member of the S 100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium bi.
Applications
- IHC - Paraffin (1:50)
- Immunofluorescence
- Western blot
- Flow Cytometry (0.5 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2836 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2836 was determined to be 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.02% Sodium Azide, BSA
Storage
Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Monkey, Rat, Bovine, Cat, Dog, Horse, Pig, Rabbit
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Species:
Human, Monkey, Mouse, Rat, Cat, Dog, Guinea pig, Pig
Applications:
IHC - Paraffin
Species:
Human, Monkey, Rat, Bovine, Cat, Dog, Horse, Pig, Rabbit
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Flow Cytometry
Species:
Human
Applications:
Western blot, Flow Cytometry
Reactivity:
Human
Range:
15.6-1000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/2/2025