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Catalog Number	Size	Price
LS-B7373-50	50 µg (0.5 mg/ml)	$485

RAMA1 / SKA3 Antibody - Anti-SKA3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

IHC‑plus™ Polyclonal Goat anti‑Human RAMA1 / SKA3 Antibody (aa311‑323, IHC) LS‑B7373

Name: IHC-plus™ RAMA1 / SKA3 Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B7373-50
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C112839

Antibody:

RAMA1 / SKA3 Goat anti-Human Polyclonal (aa311-323) Antibody

Application:

IHC, IHC-P, Peptide-ELISA

Reactivity:

Human, Dog

Format:

Unconjugated, Unmodified

$485

LS-B7373-50

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

RAMA1 / SKA3 Goat anti-Human Polyclonal (aa311-323) Antibody

Application:

IHC, IHC-P, Peptide-ELISA

Reactivity:

Human, Dog

Format:

Unconjugated, Unmodified

Specifications

Description

SKA3 antibody LS-B7373 is an unconjugated goat polyclonal antibody to SKA3 (RAMA1) (aa311-323) from human. It is reactive with human and dog. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.

Target

Human RAMA1 / SKA3

Synonyms

SKA3 | C13orf3 | RAMA1

See All RAMA1 / SKA3 Antibodies

Host

Goat

Reactivity

Human, Dog (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-PLSKTNSSSNDLE, from the internal region of the protein sequence according to NP_659498.3.

Epitope

aa311-323

Specificity

Human SKA3.

Applications

IHC
IHC - Paraffin (3.75 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:64000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 35kDa and 150kDa band in HeLa lysates after 2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 46.4kDa according to NP_659498.3. Both bands were successfully blocked by incubation with the immunizing peptide.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About RAMA1 / SKA3

Uniprot: Q8IX90 NCBI: NM_145061 NP_659498.4

Validation

Anti-SKA3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

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