IHC‑plus™ Monoclonal Mouse anti‑Human RAD50 Antibody (clone 13B3, aa1‑425, IHC, IF, WB) LS‑B1708
IHC‑plus™ Monoclonal Mouse anti‑Human RAD50 Antibody (clone 13B3, aa1‑425, IHC, IF, WB) LS‑B1708
Note: This antibody replaces LS-C20035
Antibody:
RAD50 Mouse anti-Human Monoclonal (aa1-425) (13B3) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Format:
Unconjugated, Unmodified
Overview
Antibody:
RAD50 Mouse anti-Human Monoclonal (aa1-425) (13B3) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Format:
Unconjugated, Unmodified
Specifications
Description
RAD50 antibody LS-B1708 is an unconjugated mouse monoclonal antibody to human RAD50 (aa1-425). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RAD50
Synonyms
RAD50 | DNA repair protein RAD50 | HRad50 | NBSLD | RAD50 (S. cerevisiae) homolog | RAD50 homolog (S. cerevisiae) | RAD502 | RAD50-2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Amino acids 1-425 of Rad50 expressed in E. coli.
Specificity
Amino acids 1-425 of Rad50 expressed in E. coli.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Immunofluorescence
- Western blot
- Immunoprecipitation
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B1708 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1708 was determined to be 10 ug/ml.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Anti-RAD50 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-RAD50 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human
Applications:
Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human, Mouse
Applications:
Western blot, Immunoprecipitation
Species:
Human
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/12/2025