Target
Human PRLR / Prolactin Receptor
Synonyms
PRLR | HPRL receptor | PRL-R | HPRLrI | Prolactin receptor
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide from human PRLR / Prolactin receptor.
Specificity
Recognizes the unique sequence of the human prolactin receptor (PRLr) intermediate isoform. Reactivity is confirmed with T47D breast carcinoma cell line.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1 - 3 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2835 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2835 was determined to be 5 ug/ml. Immunoprecipitation: 10 ug/IP reaction.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRLR / Prolactin Receptor