Human PKM / Pyruvate Kinase, Muscle

Rabbit

Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the sequence of human PKM2 (KLH). Molecular Weight: 60kD.

Recognizes endogenous levels of total human PKM2 protein at 60kD. Species cross-reactivity: monkey, mouse and rat.

• IHC
• IHC - Paraffin (1:100)
• IHC - Frozen (1:100)
• Western blot (1:1000)

Immunohistochemistry: LS-B2445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2445 was determined to be 1:100.

10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14618 NCBI: NM_002654 NP_002645.3