Target
Human PACE4 / PCSK6
Synonyms
PCSK6 | PACE4 | SPC4
Reactivity
Human, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-YHTFSAHQSRSR, from the internal region of the protein sequence according to NP_002561.1NP_612192.1NP_612195.1NP_612197.1NP_612196.1NP_612198.2NP_612193.1NP_612194.1.
Specificity
Human PCSK6 / PACE4. This antibody is expected to recognize reported isoforms a, b, g and h (NP_002561.1; NP_612192.1; NP_612193.1; NP_612194.1 resp.).
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 40-45 kD band in Human Brain (Cerebellum) lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 106 kD according to NP_002561.1. The 40-45 kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee