Human p16INK4a / CDKN2A

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-HARIDAAEGPSDIPD, from the C-Terminus of protein sequence according to NP_000068.1NP_001182061.1.

aa91-105

Human CDKN2A. This antibody is expected to recognise isoform p16INK4a (NP_000068.1) and isoform p16gamma (NP_001182061.1).

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.1 - 1 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Immunohistochemistry: LS-B193 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B193 was determined to be 5 ug/ml.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.