Target
Human OPTN / Optineurin
Synonyms
OPTN | ALS12 | E3-14.7K-interacting protein | FIP-2 | HIP-7 | HYPL | Huntingtin yeast partner L | HIP7 | TFIIIA-INTP | NEMO-related protein | NRP | Optineurin | FIP2 | GLC1E
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide corresponding to aa 559-575 (GEVLPDIDTLQIHVMDC) of human, mouse and rat optineurin
Specificity
Recognizes human, mouse and rat optineurin.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:200)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B3065 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3065 was determined to be 2.5 ug/ml. Western Blot: 1:200. Incubation time of 1 hour at RT. Overnight incubations at 4uC at lower concentrations can produce optimal results.
Presentation
TBS, pH 7.4, 0.5 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee