Target
Human NUMB
Synonyms
NUMB | C14_5527 | Numb (Drosophila) homolog | S171 | Protein numb homolog | C14orf41 | H-Numb | Numb homolog (Drosophila) | Protein S171
Reactivity
Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-PFSSDLQKTFEIEL, from the C-Terminus of protein sequence according to NP_001005743.1001005744.1NP_003735NP_001005745.1.
Specificity
Human NUMB. This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
Applications
- IHC - Paraffin (5 µg/ml)
- ICC
- Western blot (0.01 - 0.03 µg/ml)
- Immunoprecipitation
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 80 kDa band observed in Mouse Brain lysates (calculated MW of 70.8 kDa according to NP_001005743.1). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee