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| Catalog Number | Size | Price |
|---|---|---|
| LS-B114-50 | 50 µg (1 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human NR4A1 / NUR77 Antibody (aa251‑266, IHC, IF, WB) LS‑B114
IHC‑plus™ Polyclonal Rabbit anti‑Human NR4A1 / NUR77 Antibody (aa251‑266, IHC, IF, WB) LS‑B114
Note: This antibody replaces LS-C762, LS-C73134
Antibody:
NR4A1 / NUR77 Rabbit anti-Human Polyclonal (aa251-266) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
NR4A1 / NUR77 Rabbit anti-Human Polyclonal (aa251-266) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
NUR77 antibody LS-B114 is an unconjugated rabbit polyclonal antibody to NUR77 (NR4A1) (aa251-266) from human. It is reactive with human, mouse and rat. Validated for ChrIP, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human NR4A1 / NUR77
Synonyms
NR4A1 | GFRP1 | Early response protein NAK1 | HMR | NGFIB | Orphan nuclear receptor TR3 | Orphan receptor tr3 | Steroid receptor TR3 | TR3 | NAK1 | NGFI-B | NP10 | Receptor NGFIB | Testicular receptor 3 | TR3 orphan receptor | N10 | NAK-1 | NUR77 | Orphan nuclear receptor HMR
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human NR4A1 / NUR77.
Epitope
aa251-266
Specificity
A synthetic peptide corresponding to aa 251-266 of human Nak1.
Applications
- IHC
- IHC - Paraffin (20 - 40 µg/ml)
- ICC
- Immunofluorescence
- Western blot (1:500 - 1:1000)
- Immunoprecipitation (1:10 - 1:500)
- Chromatin Immunoprecipitation (1:10 - 1:500)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B114 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B114 was determined to be 20-40 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NR4A1 / NUR77
Validation
Anti-NUR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-NUR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NR4A1 / NUR77 Antibody (aa251-266) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B114 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Nuclear receptor NR4A1 is a tumor suppressor down-regulated in triple-negative breast cancer. Wu H #, Bi J #, Peng Y #, Huo L #, Yu X, Yang Z, Zhou Y, Qin L, Xu Y, Liao L, Xie Y, Conneely OM, Jonkers J, Xu J,. Oncotarget. 2017 8:54364-54377. (ICC; Human)
Nur77 gene expression levels were involved in different ACTH-secretion autonomy between Cushing's disease and subclinical Cushing's disease. Tabuchi Y, Kitamura T, Fukuhara A, Mukai K, Onodera T, Miyata Y, Hamasaki T, Oshino S, Saitoh Y, Morii E, Otsuki M, Shimomura I. Endocrine Journal. 2016 June;63:545-554.
Customer Reviews (0)
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Species:
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Applications:
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Species:
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Applications:
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Species:
Human, Mouse, Rat, Hamster
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Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
ELISA, Dot Blot
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
