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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3902-50 | 50 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human NOS3 / eNOS Antibody (clone 6H2, IHC) LS‑B3902
IHC‑plus™ Monoclonal Mouse anti‑Human NOS3 / eNOS Antibody (clone 6H2, IHC) LS‑B3902
Note: This antibody replaces LS-C108797
Antibody:
NOS3 / eNOS Mouse anti-Human Monoclonal (6H2) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
NOS3 / eNOS Mouse anti-Human Monoclonal (6H2) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
ENOS antibody LS-B3902 is an unconjugated mouse monoclonal antibody to human eNOS (NOS3). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human NOS3 / eNOS
Synonyms
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
6H2
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of human eNOS expressed in E. Coli.
Specificity
Human NOS3
Applications
- IHC
- IHC - Paraffin (1:100 - 1:200)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3902 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3902 was determined to be 1:100.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NOS3 / eNOS
Validation
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
See More About...
LSBio Ratings
IHC-plus™ NOS3 / eNOS Antibody (clone 6H2) for IHC, ELISA LS-B3902 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Species:
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Applications:
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Species:
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Species:
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Applications:
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Species:
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
